Description
Ernwin (Kerpedjiev 2015) is program that performs a Metropolis-Rosenbluth
like Monte Carlo sampling of coarse grained RNA 3D structures. Lokal
conformations are sampled directly from possible conformations found in
crystal structures or structures predicted with very accurate methods. An
energy function used in the accept-reject step of the sampling procedure
accounts for long-range interactions and directs the global structure in
the desired way. Single molecule Cryo Electron Microscopy and Atomic Force
Microscopy images can show large-scale properties of RNA molecules projected
to a 2D plane. We compare such images to mathematically generated projections
of predicted RNA 3D structures to improve our structure prediction model
and to draw conclusions about the observed RNA.
